Microbe Profile: Dictyostelium discoideum: model system for development, chemotaxis and biomedical research
نویسندگان
چکیده
منابع مشابه
Ca2+ chemotaxis in Dictyostelium discoideum.
Using a newly developed microfluidic chamber, we have demonstrated in vitro that Ca(2+) functions as a chemoattractant of aggregation-competent Dictyostelium discoideum amoebae, that parallel spatial gradients of cAMP and Ca(2+) are more effective than either alone, and that cAMP functions as a stronger chemoattractant than Ca(2+). Effective Ca(2+) gradients are extremely steep compared with ef...
متن کاملAssessment of development and chemotaxis in Dictyostelium discoideum mutants.
Studies using the social amoeba Dictyostelium discoideum have greatly contributed to the current understanding of the signaling network that underlies chemotaxis. Since directed migration is essential for normal D. discoideum multicellular development, mutants with chemotactic impairments are likely to have abnormal developmental morphologies. We have used multicellular development as a readout...
متن کاملDictyostelium discoideum chemotaxis: threshold for directed motion.
The chemotactic response of Dictyostelium discoideum cells to stationary, linear gradients of cyclic adenosine 3',5'-monophosphate (cAMP) was studied using microfluidic devices. In shallow gradients of less than 10(-3) nM/microm, the cells showed no directional response and exhibited a constant basal motility. In steeper gradients, cells moved up the gradient on average. The chemotactic speed a...
متن کاملNavigating signaling networks: chemotaxis in Dictyostelium discoideum.
Studies of chemotaxis in the social amoeba Dictyostelium discoideum have revealed numerous conserved signaling networks that are activated by chemoattractants. In the presence of a uniformly distributed stimulus, these pathways are transiently activated, but in a gradient they are activated persistently and can be localized to either the front or the back of the cell. Recent studies have begun ...
متن کاملSelection of chemotaxis mutants of Dictyostelium discoideum
A method has been developed for the efficient selection of chemotaxis mutants of Dictyostelium discoideum. Mutants defective in the chemotactic response to folate could be enriched up to 30-fold in one round of selection using a chamber in which a compartment that contained the chemoattractant was separated by a sandwich of four nitrocellulose filters from a compartment that contained buffer. M...
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ژورنال
عنوان ژورنال: Microbiology
سال: 2021
ISSN: 1350-0872,1465-2080
DOI: 10.1099/mic.0.001040